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OPTIZYME™ M-MLV Reverse Transcriptase, Fisher BioReagents™
72.45 EUR - 148.40 EUR
Specifications
| Concentration | 200 U/μL |
|---|---|
| Color | Colorless |
| pH | 8.3 |
| For Use With (Application) | Generation of labeled cDNA probes for microarrays, synthesis of cDNA for cloning and expression, analysis of RNA by primer extension, DNA labeling, optimum activity at 42°C, incorporates modified Nucleotides |
| Storage Buffer | OPTIZYME™ M-MLV RT Buffer 5X : Tris HCl 250mM (8.3 pH at 25°C), KCl 250mM, MgCl2 20mM, DTT 50mM |
Description
OPTIZYME™ M-MuLV RT is a genetically modified MLV RT that possesses RNA- and DNA-dependent polymerase activity.
Applications and Features:
- OPTIZYME M-MLV RT is used for efficient first strand cDNA synthesis for RT-PCR and real-time RT-PCR (up to 13kb)
- Generation of labeled cDNA probes for microarrays
- Synthesis of cDNA for cloning and expression
- Analysis of RNA by primer extension
- DNA labeling
- Optimum activity at 42°C (active up to 50°C)
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Supplied with:
5X OPTIZYME M-MLV RT Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 20mM MgCl2, 50mM DTT
Source: E. Coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase
Quality Control:
- Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free
- Functionally tested in first strand cDNA and RT-PCR
Specifications
| 200 U/μL | |
| 8.3 | |
| OPTIZYME™ M-MLV RT Buffer 5X : Tris HCl 250mM (8.3 pH at 25°C), KCl 250mM, MgCl2 20mM, DTT 50mM | |
| E. coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus Reverse Transcriptase | |
| Liquid |
| Colorless | |
| Generation of labeled cDNA probes for microarrays, synthesis of cDNA for cloning and expression, analysis of RNA by primer extension, DNA labeling, optimum activity at 42°C, incorporates modified Nucleotides | |
| -20°C | |
| OPTIZYME™ M-MLV Reverse Transcriptase |
Safety and Handling
Product Identifier
- OPTIZYME