missing translation for 'onlineSavingsMsg'
Learn More

OPTIZYME™ XhoI, Fisher BioReagents™

Brand: Fisher BioReagents BP8010-1

Product Code. 11914852

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
View alternative products »

This item is not returnable. View return policy

Description

5'....C^T C G A G...3'
3'....G A G C T^C...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl₂ , 100mM KCl and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
Ligation and Re-cleavage:
More than 95% of DNA fragments can be ligated and re-cut after a 50-fold over-digestion with XhoI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of XhoI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Note:
Supercoiled plasmids may require up to 5-fold more XhoI for complete digestion than linear DNA.
Compatible Ends: BauI, Eco88I, SalI, SmoI
Methylation Effects:
Dam: Never overlaps - no effect
Dcm: Never overlaps - no effect
CpG: Completely overlaps - cleavage impaired
CTCGAG

(Cleavage impaired)
EcoKI: Never overlaps - no effect
EcoBI: Never overlaps - no effect

Specifications

Concentration	10 U/μL
Incubator Temperature	37°C
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Xhol
Quantity	3000 U
Product Type	XhoI

Components	10X OPTIZYME™ Buffer 5
pH	7.4
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Cut Site	C.TCGAG
Form	Liquid

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier