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Thermo Scientific™ Kligler Iron Agar (Dehydrated)
Identify Enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production with this medium.
Brand: Thermo Scientific™ CM0033B
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Description
Identify Enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production using Thermo Scientific™ Oxoid™ Kligler Iron Agar (Dehydrated). Klingler Iron Agar combines the features of Kligler's Lead Acetate medium1,2,3 and Russell's Double Sugar Agar4. The medium is recommended for the identification of colonies picked off from plating media such as MacConkey Agar, Bismuth Sulphite Agar, or Desoxycholate Citrate Agar, etc.
Differentiate Enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production with Klinger Iron Agar.
Non lactose fermenters initially produce a yellow slant and butt as a result of glucose fermentation. Once the glucose is depleted, the reactions reverts to red slants (alkaline) due to oxidation of acids. Lactose fermenting organisms produce yellow slants and butts. There is no reversion to red in the slant because enough acid is produced to maintain an acid pH under aerobic conditions. Non-fermenters produce red slants and butts. H 2 S production results in a blackening of the medium, either throughout the butt or in a ring formation near the top of the butt. Gas production is demonstrated by the presence of bubbles or cracks in the medium.
Typical reactions observed on Kligler Iron Agar after 18-24 hours at 35°C.
| Organism | Slope | Butt | Gas | H2S |
| Shigella sonnei | Red | Yellow | - | - |
| Shigella dysenteriae | Red | Yellow | - | - |
| Salmonella typhi | Red | Yellow | - | + |
| Salmonella species | Red | Yellow | + | + |
| Enterobacter species | Red | Yellow | + | - |
| Klebsiella species | Yellow | Yellow | + | - |
| Escherichia coli | Yellow | Yellow | + | - |
| Proteus mirabilis | Red | Yellow | - | + |
| Morganella species | Red | Yellow | V | - |
| Citrobacter freundii | Yellow | Yellow | + | + |
| Yersinia species | Red | Yellow | V | - |
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
Specifications
| Kligler Iron Agar | |
| Agar | |
| For 9.1L medium |
| Powder | |
| 500 g |
For Laboratory Use Only