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  5. Thermo Scientific Chemicals Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln., pH 5.2

Thermo Scientific Chemicals Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln., pH 5.2

Product Code. 15495795
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Quantity:
100 mL
400 mL

Description

Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids.

Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description
• This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0
Applications
• Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA and RNA
• When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
• It is also used to generate DNA minisatellite fingerprints
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 136112-00-0
Molecular Formula C12H19Cl3O2
Molecular Weight (g/mol) 301.632
MDL Number MFCD00133763
InChI Key ZYWFEOZQIUMEGL-UHFFFAOYSA-N
Synonym phenol-chloroform-isoamyl alcohol mixture, chloroform; isoamyl alcohol; phenol, phenol chloroform isoamyl alcohol, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 125:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, vetec tm reagent grade, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 49.5:49.5:1, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 5.2, with alkaline buffer, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 6.7, with alkaline buffer
PubChem CID 66587205
IUPAC Name chloroform;3-methylbutan-1-ol;phenol
SMILES CC(C)CCO.C1=CC=C(C=C1)O.C(Cl)(Cl)Cl

Specifications

Color Colorless
UN Number UN2922
Solubility Information Soluble in water.
pH 5.2
Quantity 100 mL
Physical Form Liquid
Chemical Name or Material Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln.

Safety and Handling

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Product Identifier
  • Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 3
  • Carcinogenicity Category 2
  • Serious eye damage/eye irritation Category 1
  • Germ cell mutagenicity Category 2
  • Reproductive toxicity Category 2
  • Skin corrosion/irritation Category 1 B
  • Specific target organ toxicity Category 1
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H301-Toxic if swallowed.
  • H311-Toxic in contact with skin.
  • H314-Causes severe skin burns and eye damage.
  • H318-Causes serious eye damage.
  • H331-Toxic if inhaled.
  • H336-May cause drowsiness or dizziness.
  • H341-Suspected of causing genetic defects.
  • H351-Suspected of causing cancer.
  • H361d-Suspected of damaging the unborn child.
  • H372-Causes damage to organs.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
 Supplemental information
  • MIXTURE LIST-Contains : Chloroform, Phenol,Isoamyl alcohol

RUO – Research Use Only

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