Thermo Scientific™ Pierce™ Iminobiotin Agarose

Description

Thermo Scientific™ Pierce Iminobiotin and Biotin Resins provide for unusual affinity purification experiments involving clean-up or removal of streptavidin or avidin protein components.

Pierce Biotin Agarose and Iminobiotin Agarose are beaded agarose supports onto which biotin and iminobiotin moieties have been covalently immobilized. Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample. Occasionally, however, the reverse situation arises in which immobilized biotin in needed to capture avidin or streptavidin components from a sample. These biotin and iminobiotin resins address this special situation.

Immobilized biotin binds avidin and streptavidin very strongly, making it appropriate for non-reversible binding applications. Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.

Highlights:

• Enable purification or removal of avidin or streptavidin samples
• Resins are crosslinked beaded agarose onto which biotin or iminobiotin are covalently immobilized
• Use Biotin Agarose for non-reversible binding (e.g., removal of avidin components from a sample)
• Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)