Thermo Scientific™ Krypton™ Fluorescent Protein Stain

Description

Thermo Scientific™ Pierce Krypton Fluorescent Protein Stain enables sensitive fluorescent visualization (520nm excitation; 580nm emission) of proteins separated by polyacrylamide gel electrophoresis or on transfer membranes using popular commercial fluorescence imagers.

This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane (greater than 2ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.

Highlights:

• Intense fluorescent stain – excitation and emission maxima of 520nm and 580nm, respectively (compatible with 532nm excitation light sources and 580 to 600nm emission filters)

• Instrument-compatible – effective with popular laser-based and filter-based fluorescence imagers

• Broad dynamic range – linear quantitative range spans three to four orders of magnitude

• Sensitive – detect as little as 0.25ng protein with the standard protocol (approx. 2.5 hours); at least as sensitive as fluorescent stains from other suppliers

• Fast – standard (2.5 hours) and rapid (30 minutes) protocols are significantly faster than procedures with other popular fluorescent stains

• Versatile – compatible with proteomics workflows involving downstream mass spectrometry

• Compact – supplied as a 10X solution to save storage space; just dilute with water before use