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Thermo Scientific™ Micrococcal Nuclease Solution (≥100 U/μL)
Remove nucleic acids from cell lysates, release chromatin-bound proteins and shear chromatin for chromatin immunoprecipitation (ChIP) using micrococcal nuclease.
Brand: Thermo Scientific™ 88216
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Description
Thermo Scientific™ Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
This micrococcal nuclease is a stable liquid form of the enzyme derived from Staphylococcus aureus. Micrococcal nuclease (MNase) exhibits exo- and endo-5'-phosphodiesterase activities against DNA and RNA. This enzyme digests double-stranded, single-stranded, circular and linear nucleic acids. The highest activity is toward single-stranded nucleic acid substrates with preference for AT- or AU-rich regions. Enzymatic activity occurs at pH 7 to 10 and is strictly dependent on calcium for the digestion of RNA and DNA substrates. Micrococcal nuclease is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.Highlights:
- Digests nucleic acids (DNA and RNA)
- Effective for double-stranded, single-stranded, circular and linear nucleic acids
- Active in neutral to alkaline buffer conditions containing divalent calcium ions
- Supplied at ≥100 units/μL in 10mM Tris-HCl, pH 7.5, 50mM NaCl, 1mM EDTA, 50% glycerol
Compatible with:
Thermo Scientific Subcellular Protein Fractionation Kit (Part No. 78840); Thermo Scientific Pierce ChIP Kit (Part No. 26156)
Recommended for:
Enzymatic DNA shearing for chromatin immunoprecipitation assays (ChIP); Removal of unwanted genomic or organellar nucleic acids from cell lysates; Degradation of nucleic acids in subcellular fractionation procedures
Specifications
| Cell Lysis Enzyme | |
| Liquid | |
| 150 μL |
| Upon receipt store at -20°C. Product is shipped with dry ice. | |
| Enzyme for Cell Lysis |
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Product Title
Thermo Scientific™ Micrococcal Nuclease Solution (≥100 U/μL) > Enzyme; 150μL
For Research Use Only. Not for use in diagnostic procedures.
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