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Thermo Scientific™ Pierce™ Fluorescent Protease Assay Kit
Determine total protease activity in biological samples via fluorescent or colorimetric detection by measuring digestion of labeled casein substrate.
Brand: Thermo Scientific™ 23266
295.76 EUR valid until 2024-11-29
Use promo code "21683" to get your promotional price.
Description
Thermo Scientific™ Pierce Protease Assay Kits measure total protease activity in samples, providing a means of assessing the progress of protease isolation procedures or quantifying protease contamination in biological samples.
These Colorimetric and Fluorescent Protease Assay Kits comprise two different methods for measuring total protease activity in biological samples. Both kits can be used to assess the progress of protease purification procedures or to quantify protease contamination in protein samples. The kits use succinylated or fluorescein-tagged casein as a protease substrate. Proteolysis of the substrate results in peptides whose amino groups or altered fluorescence properties enable detection in the respective kits. Protease activity is quantified by comparison to trypsin standard included in each kit.Highlights:
Casein substrate – measure activity of any protease that cleaves casein into peptide fragments
Trypsin standard – quantify protease activity relative to trypsin, a universally accepted reference
Sensitive – 1000 times more sensitive than assays that use unmodified forms of casein
Simple and fast – test tube and microplate protocols are completed in less than one hour
Homogenous – addition steps only; no separation, transfer or stop steps required
Customizable – easily adapt time, temperature and pH to optimize sensitivity for proteases of interest
- Substrate: succinylated casein (not available separately)
- Basis of assay: detection of primary amines resulting from proteolysis
- Detection reagent: trinitrobenzene sulfonic acid (TNBSA)
- Measurement: absorbance at 450nm (plate reader or spectrophotometer)
- Substrate: fluorescein-labeled casein (FITC-casein); available separately as Part No. 23267
- Basis of assay: change in fluorescence resonance (FRET) or fluorescence polarization (FP)
- Measurement: instrument with fluorescein excitation and emission filters (485/538nm)
Includes:
Modified casein, trypsin standard and Tris-buffered saline (both kits); TNBSA solution (Part No. 23263 only)
Recommended for:
Assess functional integrity and overall progress of protease purification; Quantify protease contamination in protein samples; Characterize the activity of unusual proteases compared to trypsin; Evaluate buffer conditions for their affects on protease function and activity
Specifications
Sufficient For: 2000 microplate assays using an instrument fitted with a fluorescein filter set (ex/em 485 nm/538 nm) • FTC-Casein, lyophilized, 2.5 mg • TPCK Trypsin Standard, 50 mg • TBS Buffer Pack (makes 500 mL), 1 pack Store FTC-Casein and TPCK Trypsin at 4°C. |
|
Fluorescence | |
Classic Dye | |
Protein Activity Assay | |
Protein-Based Substrate | |
2000 Microplate Assays | |
Microplate Reader | |
Proteases |
Protease Assay | |
96-well Plate | |
Pierce™ | |
Casein | |
Protease Substrate | |
Fluorescence Polarization, FRET | |
2000 Test Kit |
For Research Use Only. Not for use in diagnostic procedures.