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Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

5.53 EUR - 1532.00 EUR

Specifications

Format Tube
GC-Rich PCR Performance High
For Use With (Application) Hot-start PCR
Concentration 2X
Fidelity (vs. Taq) 1X
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Products 7
Product Code Brand No. of Reactions Color Price Quantity & Availability  
Product Code Brand No. of Reactions Color Price Quantity & Availability  
15929082
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Invitrogen™
14001011
20 Reactions Green
5.53 EUR
Each
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15815066
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Invitrogen™
14001012
50 Reactions Green
85.75 EUR
50 reactions
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15933592
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Invitrogen™
14001013
200 Reactions Green
334.00 EUR
Each
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15939082
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Invitrogen™
14001014
1000 Reactions Green
1526.00 EUR
Each
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15889844
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Invitrogen™
14000012
50 reactions Colorless
85.75 EUR
50 reactions
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15809854
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Invitrogen™
14000014
1000 reactions Colorless
1532.00 EUR
1000 reactions
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15899844
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Invitrogen™
14000013
200 Reactions Colorless
334.00 EUR
200 reactions
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Description

Description

Invitrogen Platinum II Hot-Start PCR Master Mix (2X), available in colorless and green formats, offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase and leading hot-start technology. This master mix is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Platinum II Hot-Start Green PCR Master Mix also includes two tracking dyes for direct loading of PCR products on gels.

Features

  • Innovative buffer enables universal annealing temperature by isostabilizing primer-template duplex structures
  • Engineered Taq DNA polymerase confers fast cycling and resistance to common inhibitors
  • Hot-start technology enables superior specificity, sensitivity, and yields and allows for room temperature reaction setup
  • Green PCR buffer reduces pipetting errors with direct gel loading

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • Amplification and improved yields of GC-rich targets
  • RT-PCR
  • Genotyping
  • High-throughput PCR

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and delivers PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Hot-Start PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Notes

  • For applications that require PCR product analysis by absorbance or fluorescence excitation, colorless format is recommended.
Specifications

Specifications

Tube
Hot-start PCR
1X
5 kb or less
High
2X
Built-In Hot Start
Videos
Safety and Handling

Safety and Handling

missing translation for 'shelfLife' : 24 Months

SDS
Documents

Documents

Product Certifications

For Research Use Only.