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R&D Systems™ Recombinant Human Wnt-3a Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Brand: R&D Systems™ 5036-WN-010/CF
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Description
Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 5.00-25.0 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.
Specifications
| Lyophilized from a 0.2μm filtered solution in PBS, EDTA and CHAPS. | |
| 37.4 kDa | |
| 10 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution. | |
| Recombinant |
| 89780 | |
| Wnt-3a | |
| Chinese Hamster Ovary cell line,CHO-derived human Wnt-3a protein Ser19-Lys352 | |
| Unconjugated | |
| 75%, by SDS-PAGE with silver staining. |
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