Thermo Scientific™ Remel™ BactiDrop™ Oxidase

Description

Detect cytochrome oxidase enzyme with Thermo Scientific™ Remel™ BactiDrop™ Oxidase. The oxidase test was originally devised to identify Neisseria species, but later was used to separate the Pseudomonadaceae from the oxidase-negative Enterobacteriaceae1,2. Most gram-positive bacteria are oxidase-negative and many of the gram-negative bacilli, other than the Enterobacteriaceae, are variable3. This formulation is based on the oxidase reagent formula of Kovacs1.

Detect cytochrome oxidase enzyme with BactiDrop™ Oxidase.Ready for use: No further preparation is required.Easy to interpret:Positive test: Development of a violet to purple color within 10-30 seconds.Negative test: Delayed reaction or no color development within 10-30 seconds.BactiDrop Oxidase is used to detect cytochrome oxidase enzyme. The oxidase test is based on the bacterial production of an intracellular oxidase enzyme. The oxidase reaction is due to the presence of a cytochrome oxidase system, which activates the oxidation of reduced cytochrome by molecular oxygen, which in turn acts as an electron acceptor in the terminal stage of the electron transfer system. Microorganisms which produce oxidase enzyme, when in the presence of atmospheric oxygen, cytochrome c, and phenylenediamine oxidase reagent, oxidize to form a purple colored compound, indophenol. Ascorbic acid is incorporated in the reagent as a reducing agent to reduce auto-oxidation and to improve stability3.BactiDrop Oxidase contains N,N,N,N-tetramethyl-1,4-phenylenediamine (CAS 100-22-1), ascorbic acid (CAS 50-81-7) and demineralized water (CAS 7732-18-5). The reagent is supplied as 0.75 mL ampules.This product is for in vitro diagnostic use. This reagent has been tested using quality control procedure and found to be acceptable.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.