Learn More
Ambion™ RiboMinus™ Eukaryote Kit for RNA-Seq
The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis.
Brand: Ambion™ A1083708
1212.80 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Description
The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.
The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:
• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology
Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module (Cat. No. K155005).
Specifications
Each kit contains: |
|
Total RNA | |
High-throughput Compatible | |
8 Preps | |
1 Each | |
Wet Ice | |
Transcriptome RNA | |
1 μg |
Magnetic Bead | |
Transcriptome RNA | |
Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction | |
RiboMinus™ | |
Transcriptome Sequencing | |
2-10 μg Total RNA | |
RiboMinus |
For Research Use Only. Not for use in diagnostic procedures.