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Invitrogen™ RiboMinus™ Pan-Prokaryote Probe Mix

Product Code. 17125792
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Product Code. 17125792

Brand: Invitrogen™ A46920

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The RiboMinus™ Pan-Prokaryote Probe Mix is a pool of more than 150 3'-biotin labeled ssDNA oligonucleotides that are used to capture and eliminate bacterial and archaebacterial ribosomal RNA from RNA samples in preparation for NGS transcriptome studies.

RiboMinus™ Pan-Prokaryote Probe Mix is a component of the RiboMinus Bacteria 2.0 Transcriptome Isolation Kit and is available as a standalone product for use in conjunction with other RiboMinus products targeting other species: Plant (Cat. No. A1083808) and Eukaryote v2 (Cat. No. A15026). Combining RiboMinus Pan-Prokaryote Probe Mix with Plant or Eukaryote probe mixes is useful for metatranscriptomic studies. This probe mix consists of over 150 3’-biotin-labeled ssDNA oligonucleotide designs. These designs were created using highly conserved regions of rRNA across 76 pan-prokaryote species (see table below) and tested with several species (see compatibility list below).
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage RiboMinus Isolation kits include RiboMinus Magnetic Beads, RiboMinus human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water. Store the isolation modules at +4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored.
Isolation Technology Magnetic Bead
Purification Time 1.25 hr.
Sample Type Total RNA (Bacterial)
Final Product Type Transcriptome RNA
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) Next Generation Sequencing, Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
No. of Reactions 12
Product Line RiboMinus
Quantity 1
Starting Material Amount 100 ng to 5 ug of total RNA
Target Transcriptome RNA
System Type RiboMinus
Form Magnetic Bead
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For Research Use Only. Not for use in diagnostic procedures.

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