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IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector
Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA Lifesciences GmbH Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
Brand: IBA Lifesciences 5-5200-001
Beschreibung
pDSG-IBA vectors for MEXi mammalian expression system
- Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
- Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
- Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number
Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Spezifikation
Constitutive | |
CMV | |
Geneticin™ (G-418) | |
Mammalian | |
Direct Cloning | |
pENTRy-IBA51 Reverse Sequencing | |
pDSG-IBAwt1 |
Ampicillin | |
Vector | |
Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage | |
Untagged | |
250 ng/μL | |
5 μg | |
Vector for recombinant expression in mammalian cells |
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