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IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination.

Brand: IBA Lifesciences 5-4967-001

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Product Code. 15673848

266.00 EUR / 5µg

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Description

pLSG-IBA Baculo virus (insect cells) expression vectors

The pLSG-IBAwt1 vector carries the Polyhedrin promoter for high-level expression in insect cells.

Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild type virus to great extent.
Cloning and expression of recombinant proteins in insect cells
Polyhedrin promoter drives high-level expression of the insert
Ampicillin resistance and ColE1 origin of replication (pUC) support propagation in E. coli

Please note that cloning into pLSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pLSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Format	Liquid
Description	pLSG-IBA 167
Includes	pUC ori, Ampicillin resistance
Promoter	Polyhedrin
Storage Buffer	20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Protein Tag	Twin-Strep-tag/FLAG-tag for N-terminal
Quantity	5 μg
For Use With (Application)	Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins

For Use With (Equipment)	StarGate™ System, Insect Host Cells
Inducing Agent	BacPAK6 linearized A cmNPV DNA (Clontech) or circular flashBAC modified A cmNPV DNA (Oxford Expression Technologies)
pH	8
Product Type	Acceptor Vector for bacula (insect cell) expression
Content And Storage	2°C to 8°C for frequent usage, -20°C for long-term storage
Concentration	250 ng/μL
Color	Clear

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IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo

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