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Merck Millipore Sterikon™ plus Bioindicator
For monitoring the effectiveness of steam sterilization. Sterikon™ plus Bioindicator ampules contain spores of an apathogenic organism that is destroyed with sufficient autoclaving. When the ampoules are incubated, incomplete sterilization is indicated by growth of the organism.
Brand: Merck Millipore 1102740002
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Description
The Sterikon™ plus Bioindicator System is used to monitor the effectiveness of steam sterilization at 121°C for 15 minutes. It consists of an ampule that contains nutrient broth, sugar, a pH indicator and spores of an apathogenic organism, Geobacillus stearothermophilus ATCC 7953 (sporulation optimized).
- Spores are totally killed after 15 min. when heated at 121°C ± 0.5°C (1 bar)
- At lower temperatures or lower exposure times, the spores can survive, at least partly
- An appropriate number of ampules are placed into the autoclave along with the batch to be sterilized
- The ampules are incubated up to 48 hours at 60 ± 2°C
- No growth of the organism indicates adequate sterilization; growth shows inadequate sterilization
