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  6. 10445345

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Brand:  Thermo Scientific™ 34075

311.34 EUR valid until 2025-03-21
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Product Code. 10445345

  • 467.00 EUR / 100mL
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Description

Description

Thermo Scientific™ SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems and quantitation. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, enabling multiple film or camera exposures.

SuperSignal West Dura Extended Duration Substrate characteristics include:

  • Sensitivity: mid-femtogram
  • Stability: 24-hr working solution stability; 1-year kit stability at room temperature
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 24 hours
  • Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
  • Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Substrate
Liquid
Nitrocellulose, PVDF
100 mL
2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL)
24 hr.
Luminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL
Chemiluminescence
Western Blot
SuperSignal™
Horseradish Peroxidase
Mid-femtogram
12 Mini-Gel Blots, 1000 sq. cm of Membrane
Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store at room temperature.
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot NumberZK400245 Certificate TypeCertificate of Analysis Date16/12/2024 Catalog Number34075,34075_LITL18 PROMO
Lot NumberZK400245A Certificate TypeCertificate of Analysis Date16/12/2024 Catalog Number34075,34075_LITL18 PROMO
Lot NumberZI397732 Certificate TypeCertificate of Analysis Date16/10/2024 Catalog Number34075,34075_LITL18 PROMO
Lot NumberZH394561 Certificate TypeCertificate of Analysis Date04/09/2024 Catalog Number34075,34075_LITL18 PROMO
Lot NumberZH394561A Certificate TypeCertificate of Analysis Date04/09/2024 Catalog Number34075,34075_LITL18 PROMO
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Product Title
Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate > West Dura Substrate; 100mL kit

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