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Thermo Scientific™ T-REx™ Tango™ GHSR-bla U2OS Cells
The T-REx™ Tango™ GHSR-bla U2OS cells contain the human Growth Hormone Secretagogue Receptor (GHSR) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Brand: Thermo Scientific™ K1819
This item is not returnable.
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Description
The T-REx™ Tango™ GHSR-bla U2OS cells contain the human Growth Hormone Secretagogue Receptor (GHSR) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The T-REx™ Tango™ GHSR-bla U2OS cells are functionally validated for Z' and EC50 concentrations of Ghrelin. In addition, T-REx™ Tango™ GHSR-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
Specifications
| NM_198407 | |
| Dividing Cells | |
| Tail from AVPR2 GPCR | |
| Fluorescence | |
| GHSR | |
| T-REx™ Tango™ GHSR-bla U2OS cells (LiveBLAzer™-FRET B/G loading kit, solution D and other materials are required separately; please refer to the protocol). Includes: T-REx™ Tango™ GHSR-bla U2OS cells, T-REx™ Tango™ GHSR-bla U2OS assay protocol, certificate of analysis | |
| GeneBLAzer™, Tango™ | |
| End Point | |
| GHSR | |
| U2OS | |
| GPCR |
| Cell-based beta-arrestin recruitment | |
| LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • T-REx™ Tango™ GHSR-bla U2OS cells • T-REx™ Tango™ GHSR-bla U2OS Assay Protocol • Certificate of Analysis The T-REx™ Tango™ GHSR-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
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| T-REx™ Tango™ GHSR-bla U2OS Cells, 8 x 106 Cells, U2OS Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage | |
| FRET | |
| G-Protein Coupled Receptor (GPCR) | |
| Single Cell Clone Origin | |
| 8 x 106 Cells | |
| U2OS | |
| U2OS Cell | |
| Tango™ |
For Research Use Only. Not for use in diagnostic procedures.