missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. 10532374

Thermo Scientific™ Tango™ CCR6-bla U2OS DA Assay Kit

The Tango™ CCR6-bla U2OS cells contain the human Chemokine (C-C motif) Receptor 6 (CCR6) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

Brand:  Thermo Scientific™ K1765

Product Code. 10532374

  • 786.00 EUR / Each
Estimated Shipment: 20-01-2025
to see stock.
Explore more special offers
Add to basket
This item is not returnable. View return policy

Description

Description

The Tango™ CCR6-bla U2OS cells contain the human Chemokine (C-C motif) Receptor 6 (CCR6) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ CCR6-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ CCR6-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

NM_004367.3
Division-Arrested Cells
Native Tail
GPCRs
Tango™ CCR6-bla U2OS DA cells, LiveBLAzer™-FRET B⁄G Loading Kit, 70 μg, Solution D, Tango™ CCR6-bla U2OS DA Assay Protocol, LiveBLAzer™-FRET B⁄G Loading Protocol, Certificate of Analysis
Tango™
BLA (Beta-Lactamase)
Division-Arrested Cells Assay Kit
Tango™
Cell-based beta-arrestin recruitment
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• Tango™ CCR6-bla U2OS DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• Tango™ CCR6-bla U2OS DA Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The Tango™ CCR6-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Fluorescence
CCR6
Single Cell Clone Origin
8 x 106 Cells
CCR6
U2OS
GPCR
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.