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Thermo Scientific™ Tango™ F2RL1-bla U2OS Cells
The Tango™ F2RL1-bla U2OS cells contain the human Coagulation factor II (thrombin) receptor-like 1 (F2RL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Brand: Thermo Scientific™ K1830
Description
The Tango™ F2RL1-bla U2OS cells contain the human Coagulation factor II (thrombin) receptor-like 1 (F2RL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ F2RL1-bla U2OS cells are functionally validated for Z' and EC50 concentrations of SLIGRL. In addition, Tango™ F2RL1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
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Specifications
NM_005242.3 | |
Dividing Cells | |
Tail from AVPR2 GPCR | |
Fluorescence | |
F2RL1 | |
Tango™ F2RL1-bla U2OS cells (LiveBLAzer™-FRET B/G loading kit, solution D and other materials are required separately; please refer to the protocol). Includes: Tango™ F2RL1-bla U2OS cells, Tango™ F2RL1-bla U2OS assay protocol, certificate of analysis | |
GeneBLAzer™, Tango™ | |
End Point | |
F2RL1 | |
U2OS | |
GPCR |
Cell-based beta-arrestin recruitment | |
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ F2RL1-bla U2OS cells • Tango™ F2RL1-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ F2RL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
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Tango™ F2RL1-bla U2OS Cells, 8 x 106 Cells, ∽8,000,000 cells/vial at 8,000,000 cells/mL Quantity of Cells, U2OS Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage | |
FRET | |
G-Protein Coupled Receptor (GPCR) | |
Single Cell Clone Origin | |
8 x 106 Cells | |
U2OS | |
U2OS Cell | |
Tango™ |
For Research Use Only. Not for use in diagnostic procedures.