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Thermo Scientific™ Tango™ HTR1A-bla U2OS Cells
Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Brand: Thermo Scientific™ K1857
This item is not returnable.
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Description
Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. Tango™ HTR1A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of 5-CT (Figure 1). In addition, Tango™ HTR1A-bla U2OS cells have been tested for assay performance under variable conditions.
Specifications
| NM_000524 | |
| Dividing Cells | |
| Tail from AVPR2 GPCR | |
| FRET | |
| G-Protein Coupled Receptor (GPCR) | |
| Single Cell Clone Origin | |
| 8 x 106 Cells | |
| 5HT1A, HTR1A | |
| U2OS | |
| GPCR |
| Cell-based beta-arrestin recruitment | |
| LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ HTR1A-UAS-bla CHO-K1 cells • Tango™ HTR1A-UAS-bla CHO-K1 Assay Protocol • Certificate of Analysis The Tango™ HTR1A-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| Fluorescence | |
| HTR1A | |
| HTR1A-UAS-bla CHO-K1 cells, HTR1A-UAS-bla CHO-K1 Assay Protocol, Certificate of Analysis | |
| Tango™ | |
| End Point | |
| Dividing Cells | |
| Tango™ |
For Research Use Only. Not for use in diagnostic procedures.