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Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, without competent cells
Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products
Specifications
| Bacterial or Yeast Strain | Not Included |
|---|---|
| Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
| Cloning Method | Zero Blunt TOPO |
| Content And Storage | Box 1: Store at -5 to -30°C. • pCR XL-2-TOPO vector • Salt Solution • T3 Primer • T7 Primer • XL Control PCR Template • XL Control PCR Primers • Platinum SuperFi Green PCR Master Mix • SuperFi GC Enhancer • Nuclease-free water Box 2: Store at room temperature. • Gel Solubilization Buffer (L3) • Binding Buffer (B2) • Wash Buffer (W1) • Elution Buffer (E1) • PureLink Clean-up Spin Columns (in Wash Tubes) • PureLink Recovery/Elution Tubes |
| Format | Kit |
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|
17176458
|
Invitrogen™
K8040-10 |
10 Reactions |
N/A
|
N/A | |||||
|
17186458
|
Invitrogen™
K8040-20 |
20 Reactions |
N/A
|
N/A | |||||
Description
- The TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb).
- The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments. Amplification of long PCR fragments is enabled by Platinum™ SuperFi™ Green PCR Master Mix, which is included in the kit.
- Topoisomerase I activation of the vector allows PCR products to be ligated in just 5 minutes on your bench–top, resulting in high cloning efficiency (up to 90% positive recombinants with 10 kb fragments).
- ccdB gene for positive selection
- EcoR I site flanking the PCR product insertion site for easy excision of inserts
- Ampicillin- and kanamycin-resistance genes for your choice of selection in E. coli
- T7 promoter/priming site for in vitro RNA transcription and sequencing
- T7, T3, and M13 forward and reverse primer sites for sequencing
- TOPO XL-2 PCR Cloning Kits containing the pCR-XL-2-TOPO vector
- Platinum SuperFi Green PCR Master Mix—featuring a proofreading DNA polymerase (>100X fidelity compared to Taq) with high processivity and a density gradient to generate accurate, long PCR amplicons ready to load onto an agarose gel for gel extraction
- PureLink™ Quick Gel Extraction and PCR Purification Combo Kit—designed to purify DNA fragments from agarose gels or direct PCR purification using a silica-based spin cartridge in < 30 minutes
Specifications
| Not Included | |
| Zero Blunt TOPO | |
| Kit | |
| TOPO™ | |
| T3, T7 | |
| pCR-XL-2-TOPO |
| Ampicillin (AmpR), Kanamycin (KanR) | |
| Box 1: Store at -5 to -30°C. • pCR XL-2-TOPO vector • Salt Solution • T3 Primer • T7 Primer • XL Control PCR Template • XL Control PCR Primers • Platinum SuperFi Green PCR Master Mix • SuperFi GC Enhancer • Nuclease-free water Box 2: Store at room temperature. • Gel Solubilization Buffer (L3) • Binding Buffer (B2) • Wash Buffer (W1) • Elution Buffer (E1) • PureLink Clean-up Spin Columns (in Wash Tubes) • PureLink Recovery/Elution Tubes |
|
| PCR Cloning | |
| Cloning Kit | |
| Approved for shipment at Room Temperature or on Wet or Dry Ice |
For Research Use Only. Not for use in diagnostic procedures.