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Invitrogen™ TrueTag™ Donor DNA Kit, RFP
The Invitrogen™ TrueTag™ Donor DNA Kit, RFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.
Brand: Invitrogen™ A42993
542.08 EUR valid until 2024-09-27
Use promo code "21650" to get your promotional price.
Description
The Invitrogen TrueTag Donor DNA Kit, RFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.
Benefits of the TrueTag Donor DNA Kit include:
- One-step PCR amplification to generate donor templates—no cloning required
- Generate transfection-ready DNA donors in hours instead of days
- Enrich up to 100% GFP-tagged cells
The TrueTag Donor DNA Kit, RFP, provides everything needed to produce a high-quality DNA donor to tag genes with a selection marker and GFP. Simply provide two locus-specific primers to add homology arms, PCR-amplify, and purify for a transfection-ready donor in hours, not days. Each kit is designed to enable users to:
- Tag the carboxy- (C-) or amino- (N-) terminus of your gene of interest
- Choose from puromycin or blasticidin selection markers—whatever best suits the cell model
- Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment
Included in the kit
- Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
- Phusion Flash High-Fidelity PCR Master Mix
- PCR cleanup columns and buffers
- Positive control primers to tag human beta-actin (ACTB)
Intuitive software to rapidly design gene-specific reagents
Target-specific gene editing reagents are required for proper knock-in of the fluorescent tag. The Invitrogen TrueDesign Genome Editor web-based software was specifically created to:
- Identify target gene transcript
- Designate insertion site
- Design and enable easy ordering of all necessary components
Required reagents available via TrueDesign software (not included in kit)
- CRISPR guide RNA or TALENs designed adjacent to N- or C-terminus
- Oligos to PCR-amplify your donor and add appropriate homology arms
- Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
- Verification primers to validate proper insertion of the TrueTag DNA Donor
Also required and available for purchase separately or as part of TrueDesign workflow
- TrueCut Cas9 Protein v2, TrueCut HiFi Cas9 Protein, or GeneART CRISPR mRNA
- CRISPRMAX Transfection Reagent or MessengerMAX Transfection Reagent
Specifications
• TrueTag Assembly Kit, store at -20°C • TrueTag Cleanup Kit, store at room temperature |
|
Kit | |
RFP | |
TrueTag™ | |
Approved for shipment on Wet or Dry Ice |
Tag: RFP | |
Fluorescent gene tagging, cell line development | |
10 reactions | |
Donor DNA Kit |
For Research Use Only. Not for use in diagnostic procedures.