Molecular Probes™ TSA™ Reagent, Alexa Fluor™ 647 Tyramide

Description

New SuperBoost™ Tyramide signal Amplification Kits offer 2-10 times the sensitivity of TSA™ kits. Explore the advantages of SuperBoost™ Alexa Fluor™ 647 Tyramide Reagent, which is replacing TSA™ Reagent, Alexa Fluor™ 647 Tyramide.

This Alexa Fluor™ 647-tyramide conjugate (ex/em 650/668 nm) is a critical component of tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal (if the tyramide is conjugated to a fluorescent dye) or colorimetric detection (if the tyramide is conjugated to biotin).

For ultimate detection sensitivity, tyramide-based amplification techniques are widely used in fluorescent immunodetection and FISH procedures to visualize targets with low expression levels or suboptimal fixation. It can be used with both chromogenic and fluorescent signals on slide based assays.

In addition to the stand-alone format offered here, tyramide conjugates are also available as part of an optimized kit that includes secondary antibody-HRP conjugate or streptavidin-HRP conjugate and other essential components (view a table of all available kits). For the first-time users, we recommend use of the optimized kit. However, if the primary antibody used is not compatible with the secondary antibodies available with these kits, then stand-alone tyramide conjugates (with amplification buffer) are offered that may be used as part of a customized system.