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Gibco™ William's E Medium, no glutamine
Description
William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco™ William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.
This William's E Medium is modified as follows:
- With: Phenol Red
- Without: L-glutamine, HEPES
The complete formulation is available.
Gibco™ William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.
Dual-Site cGMP Manufacturing
Gibco™ William's E Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ William's E Medium product made in our Grand Island facility (12551-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Specifications
Specifications
| Cell Type | Mammalian Cells |
| Form | Liquid |
| Product Type | William's E Medium |
| With Additives | Phenol Red, Sodium Pyruvate |
| Without Additives | No Glutamine, No HEPES |
| For Use With (Application) | Mammalian cell culture |
| Quantity | 10 x 500 mL |
| Content And Storage | Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Culture Type | Mammalian Cell Culture |
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