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Immunoassay Reagents and Kits

Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (12,032)
Singleplex and Multiplex Bead Based Immunoassays (1,345)
ELISA Substrates and Reagents (1,230)

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1 – 15 of 11,392 results
1

Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

Promotions are available
EDGE
2

Invitrogen™ Cell Extraction Buffer

Suitable for use in ELISA and Western blotting

Promotions are available
EDGE
3

Invitrogen™ IL-6 Human ELISA Kit

Measure levels of human interleukin-6 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Promotions are available
EDGE
Measurement Absorbance at 450nm minus absorbance at 550nm
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Specificity No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6
Description Human IL-6 ELISA Kit
Precision Intra-assay CV <10%; inter-assay CV <10%
Total Assay Time 3 hr.
4

Invitrogen™ VEGF Human ELISA Kit

Quantifies natural or recombinant human Vascular Endothelial Growth Factor (VEGF) in serum, plasma, buffered solution, or cell culture medium

Promotions are available
EDGE
Target Species Human
Conjugate Biotin
Interassay CV 8.1%
Intraassay CV 4.7%
Incubation Time 4 hr.
Gene Symbol MVCD1,VEGF,VPF
Total Assay Time 4 hr.
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Assay Sensitivity <5 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 23.4-1500 pg/mL
Marker Gene VEGF
For Use With (Application) Colorimetric Detection
Test Time 1 hr. 20 min.
Accession Number P15692
Regulatory Status RUO
Target Human
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 7422
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
5

Invitrogen™ TNF alpha Human Uncoated ELISA Kit

Uncoated ELISA Kit

Promotions are available
EDGE
Target Species Human
Conjugate Biotin
Gene Symbol CANDF6,IL-17F,ML-1,ML1
Total Assay Time 24 hr. 30 min.
Sample Volume Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Assay Sensitivity 4 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 4-500 pg/mL
For Use With (Application) ELISA
Test Time 1 hr. 45 min.
Accession Number P01375
Regulatory Status RUO
Target Human
Kit Contents Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 7124
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Tumor Necrosis Factor alpha, tnf-a, tnf-alpha
6

Invitrogen™ IL-6 Human Uncoated ELISA Kit

Uncoated ELISA Kit

Promotions are available
EDGE
Target Species Human
Conjugate Biotin
Gene Symbol DER4,Fit-1,Ly84,ST2L,St2,St2-rs1,T1,T1/ST2
Total Assay Time 24 hr. 30 min.
Sample Volume Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Assay Sensitivity 2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 2-200 pg/mL
For Use With (Application) ELISA
Test Time 1 hr. 45 min.
Accession Number P05231
Regulatory Status RUO
Target Human
Kit Contents Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme:Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 3569
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin-6
7

Invitrogen™ IL-8 Human Uncoated ELISA Kit

Uncoated ELISA Kit

Promotions are available
EDGE
Target Species Human
Conjugate Biotin
Gene Symbol GMCSF
Total Assay Time 24 hr. 30 min.
Sample Volume Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Assay Sensitivity 2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 2-250 pg/mL
For Use With (Application) ELISA
Test Time 1 hr. 45 min.
Accession Number P10145
Regulatory Status RUO
Target Human
Kit Contents Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards.,96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 3576
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin-8, CXCL8, NAP-I
8

Invitrogen™ IL-18 Human ELISA Kit

For the quantitative detection of human IL-18.

Promotions are available
EDGE
Target Species Human
Conjugate Biotin
Interassay CV 8.1%
Intraassay CV 6.5%
Gene Symbol IGIF,IL-18,IL-1g,IL1F4
Total Assay Time 3 hr. 10 min.
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Assay Sensitivity 9.0 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 78-5,000 pg/mL
For Use With (Application) ELISA
Test Time 1 hr. 20 min.
Accession Number Q14116
Regulatory Status RUO
Target Human
Kit Contents Pre-coated 96 well plate,Adhesive Plate Covers,Sample Diluent,Assay Buffer concentrate,Assay Buffer concentrate,SAV-HRP,SAV-HRP,Wash Buffer,Chromogen,Chromogen,Stop Solution
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 3606
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin-18, Interferon-gamma-inducing factor
9

Invitrogen™ Cortisol Competitive Human ELISA Kit

Competitive ELISA

Promotions are available
Target Species Human
Conjugate HRP
Description Cortisol Competitive ELISA Kit
Interassay CV 8.1%
Intraassay CV 8.8%
Total Assay Time 1 hr. 30 min.
Assay Sensitivity 17.3 pg/mL
Storage Requirements -20°C
Research Discipline Inflammation
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time 1 hr. 20 min.
Detection Method Fluorescence
Regulatory Status RUO
Target Human
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Sample Type Fecal Extract,Plasma,Saliva,Serum,Supernatant,Urine
Label or Dye HRP
10

Invitrogen™ Glucose Colorimetric Detection Kit

Assay Glucose levels in Serum, Plasma, Urine, Buffers and Tissue Culture Media.

Promotions are available
Shipping Condition Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format Colorimetric Detection Kit
Content And Storage 96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet.

Store at –20°C.
For Use With (Equipment) Microplate Reader
Product Type Glucose Colorimetric Detection Kit
Gene ID (Entrez) Non-Gene
Sample Type Other Biological Fluids,Plasma,Serum
Gene Symbol Non-Gene
Target Kit Named Glucose
Detection Method Colorimetric
11

Invitrogen™ PSA (Total)/KLK3 Human ELISA Kit

Sandwich ELISA Kit

Protein Family Other Proteins
Target Species Human
Conjugate Biotin
Interassay CV <12%
Intraassay CV <10%
Gene Symbol KLK3
Total Assay Time 4 hr. 45 min.
Assay Sensitivity 8 pg/mL
Storage Requirements 2°C to 8°C
Research Discipline Angiogenesis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr. 20 min.
Assay Solid-phase sandwich ELISA
Accession Number P07288
Regulatory Status RUO
Target Human
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 354
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym APS, KLK2A1, PSA, hK3
12

Invitrogen™ Human IL-35 ELISA Kit

For the quantitative detection of Human IL-35

13

Invitrogen™ Thyroxine (T4) Competitive ELISA Kit

Competitive ELISA

Promotions are available
Target Species Human
Conjugate HRP
Description Thyroxine (T4) Competitive ELISA Kit
Interassay CV 7%
Intraassay CV 3%
Total Assay Time 1 hr. 30 min.
Assay Sensitivity 0.29 ng/mL
Storage Requirements -20°C
Research Discipline Metabolism
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time 1 hr. 20 min.
Detection Method Fluorescence
Regulatory Status RUO
Target Chemical
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Sample Type Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye HRP
14

Invitrogen™ Triiodothyronine (T3) Competitive ELISA Kit

Competitive ELISA

Target Species Human
Conjugate HRP
Description Triiodothyronine (T3) Competitive ELISA Kit
Interassay CV 13.4%
Intraassay CV 6.3%
Total Assay Time 2 hr. 30 min.
Assay Sensitivity 37.4 pg/mL
Storage Requirements -20°C
Research Discipline Metabolism
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time 1 hr. 20 min.
Detection Method Fluorescence
Regulatory Status RUO
Target Chemical
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Sample Type Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye HRP
15

Invitrogen™ TNF alpha Human ELISA Kit

Each ready-to-use kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency

Promotions are available
Target Species Human
Conjugate Biotin
Interassay CV 7.5%
Intraassay CV 4.4%
Incubation Time 4 hr.
Gene Symbol DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
Total Assay Time 4 hr.
Sample Volume Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Assay Sensitivity 1.7 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 15.6-1000 pg/mL
Marker Gene TNF-α
For Use With (Application) Colorimetric Detection
Test Time 1 hr. 20 min.
Accession Number P01375
Regulatory Status RUO
Target Human
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 7124
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP