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HPLC Columns

HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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Phase

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Material

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Technique

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1 – 15 of 657 results
1

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns
BIOPHARMA

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

EDGE
2

Thermo Scientific™ Hypersil™ BDS C8 HPLC Columns

Use as a robust, general-purpose column for QC/QA applications. They are high-quality, base-deactivated, fully endcapped, have similar C18 selectivity but slightly less retentive.

Pore Size 130 Å
Carbon Load 7%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L7
3

Thermo Scientific™ Acclaim™ 120 C18 Columns

Use these high-efficiency reversed phase C18 columns for maximum resolution in a wide variety of applications.

Pore Size 120 Å
Carbon Load 18%
Stationary Phase C18
Temperature 60°C
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 300 m²/g
USP Type L1
4

Cytiva HiTrap™ GCSFSelect

Prepacked columns specifically designed for the purification of recombinant Granulocyte-colony stimulating factor (G-CSF). Based on a highly rigid agarose base matrix that allows for high flow rates at large production scales.

Particle Size 75 μm
Ligand Type Single-Chain Antibody Fragment Directed Against G-CSF
Type Column
For Use With Purification of Recombinant Granulocyte-Colony Stimulating Factor (G-CSF)
pH 2 to 12
Quantity 5/Pk.
Purification Target Granulocyte-Colony Stimulating Factor (G-CSF)
5

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

EDGE
Carbon Load 11%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L1
6

Thermo Scientific™ NativePac OBE-1 SEC Column for Online Sample Preparation

Perform Native-MS analysis of biomacromolecules with the NativePac OBE-1 SEC Column designed for rapid exchange of non-volatile buffer components with a volatile buffer.

7

Thermo Scientific™ Syncronis™ C18 Columns

Use for hydrophobic interaction reversed-phase separations.

Pore Size 100 Å
Carbon Load 16%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 9
Surface Area 320 m²/g
USP Type L1
8

Thermo Scientific™ BioBasic™ PREP SEC 120 Size Exclusion Chromatography HPLC Columns

Achieve outstanding size separation of water soluble compounds with BioBasic™ PREP SEC 120 Size Exclusion Chromatography HPLC Columns.

Pore Size 120 Å
Stationary Phase Diol (OH)
Temperature 60°C
Phase Size Exclusion
For Use With Preparative Size Exclusion
Endcapped No
Column Type Size Exclusion
For Use With (Application) Preparative Chromatography
Surface Area 300 m²/g
Max. Pressure 4300 psi (295 bar)
Particle Size 5 μm
Carbon Load >8.2%
Packing Material Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric) 7.8 mm
pH 2 to 8
USP Type L59
9

Cytiva RESOURCE™ and SOURCE™ 15 and 5RPC columns

Designed for rapid screening experiments, method development and small-scale protein purification using reversed phase chromatography

Particle Size 15 μm
Ligand Type Matrix surface
Flow Rate 10 mL/min
Type Reversed Phase Column
Endcapped No
Packing Material Polystyrene/Divinyl Benzene
Diameter (Metric) 6.4 mm
pH 1 to 12
Material PEEK
Particle Shape Monosized
Max. Pressure 580 psi
10

Cytiva HiTrap Albumin and IgG Depletion

HiTrap Albumin and IgG Depletion is a prepacked columns for the depletion of albumin and IgG from human serum and plasma. The columns are prepacked with high performance SepharoseTM based media with an affinity for human serum albumin (HSA) and IgG

11

Thermo Scientific™ μPAC™ Neo HPLC Columns

Learn more about your proteome samples with Thermo Scientific™ μPAC™ Neo HPLC columns.

EDGE
Pore Size 100 to 300 Å
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Silicon Chip
pH 1.5 to 7
Max. Pressure 6526 psi (450 bar)
12

Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns
BIOPHARMA

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

Pore Size 100 Å
Stationary Phase C18
Phase Reversed Phase
USP Type L1
13

Hamilton™ PRP-1 Reversed Phase HPLC Columns, 10um Particle Size: Stainless Steel

Stable alternative to C8 or C18 Columns

Pore Size 100 Å
Type Column
Stationary Phase PRP-1
Temperature 5°C to 85°C
Phase Reversed Phase
Packing Material PSDVB (Polystyrene Divinylbenzene)
Column Type Reversed Phase
Mobile Phase Compatibility pH 1 to 13, 0 to 100% aqueous, organic modifier
pH 1 to 13
Material Stainless Steel
Max. Pressure 5000 psi (345 bar)
USP Type L21
Column Format Analytical Column
15

Macherey-Nagel™ Nucleosil™ C18 Reversed Phase HPLC Column, 7 μm, 4.6 mm x 250 mm

Column Format: Analytical Column; Carbon Load: 2%; Pore Size: 500 Å; Column Phase: Reversed Phase