Test
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-5 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Use for the rapid purification of DNA >10 base pairs in length in a wide range of applications. Cytiva Life Sciences™ llustra™ MicroSpin™ G-25 Columns are ideal for desalting, buffer exchange, and removal of unincorporated nucleotides from end-labeled oligonucleotides.
Purify and concentrate PCR products or DNA fragments ranging in size from 50 bp to 10 kb. Cytiva Life Sciences™ illustra™ GFX™ PCR DNA and Gel Band Purification Kit can be used to purify DNA from reaction volumes up to 100μL or agarose gel slices of up to 900mg.
Includes | GFX™ columns, collection tubes, color-coded bottles of capture Buffer, Wash Buffer, two Elution Buffers |
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Description | Designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μl or agarose gel slices up to 900 mg. |
Product Type | illustra GFX PCR DNA and Gel Band Purification Kit |
Formulation | Tris HCl and sterile Water |
Sample Type | Agarose Gel Slices,DNA Solutions,Enzyme Reactions,PCR Mixtures |
Sample Volume | 50 bp-10 kb |
Yield | PCR 10 μl elution volume-65%, PCR 50 μl elution volume-82%, 300 mg agarose 10 μl elution volume-57%, 300 mg agarose 50 μl elution volume-91% |
For Use With (Application) | Isolation and concentration of DNA fragments (50bp to 10kb) from PCR mixtures, DNA-containing Agarose Gel bands, Enzyme-based DNA modifications and restriction digestions |
Protects mRNA and improves total cDNA yields
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.
Target | Plasmid DNA |
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Format | Spin Column |
Product Type | Plasmid MiniPrep Kit |
Sample Type | Bacteria |
Isolation Technology | Silica Column |
Column Type | Spin Column |
Scale | Mini |
High-throughput Compatibility | Compatible (Vacuum manifold) |
For Use With (Application) | PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription |
Test Time | <14 min |
Product Line | GeneJET |
Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.
Shipping Condition | Room Temperature |
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Final Product Type | Total RNA, DNA, Protein |
Sample Type | Bacteria,Cells,Plants,Tissue,Yeast |
Isolation Technology | Organic Extraction |
Starting Material Amount | Bacteria: ≤107 cells Cells: ≤107 Plant: ≤107 cells, 100 mg Tissue: ≤100 mg Yeast: ≤107 cells |
Purification Time | 1 hr. |
Yield | DNA: ≤7 μg RNA: ≤73 μg Protein: varies |
Elution Volume | 20 to 600 μL |
For Use With (Application) | RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Compatible with agarose gels
Shipping Condition | Room Temperature |
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Buffer | Running Buffer |
Format | Bottle |
Product Type | TBE Buffer |
Concentration | 10 X |
For Use With (Application) | Nucleic Acid Gel Electrophoresis, Blotting |
Gel Compatibility | Polyacrylamide Gels, Agarose Gels |
Product Line | UltraPure™ |
RNAlater RNA Stabilization Solution stabilizes and protects cellular RNA in intact, unfrozen tissue samples, eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing.
Pre-mixed, pH-adjusted, sterile-filtered solution
This 1X TE Buffer is a component of the PureLink™ 96 Plasmid Purification System, now offered separately.
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
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Format | Spin Column |
Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Pre-mixed, pH-adjusted, sterile-filtered solutions
Increase the recovery of nucleic acids during preciptation with this inert carrier.