Proteomics/ Protein Biology Methods

Products for Your Workflow

Genes direct the production of proteins that play a significant role in diseases such as cancer, Alzheimer’s, and diabetes. Protein biology helps us understand the output of these genes and the way they influence our health. Through protein isolation, purification, separation, and detection, protein biology provides earlier and more accurate diagnoses.

1. Extraction
2. Clean Up
3. Purification
4. Purity Analysis
5. Protein Detection

Step 1: Extraction

Isolate proteins from your mixture with the products below. This protein biology method will help you start your process and prepare you for future workflow steps. Find protein extraction essentials, Lysis reagents, plus additional products and resources.

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Protein Extraction

Protease Inhibitors and Phosphatase Inhibitors

Dialysis and Desalting

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Step 2: Clean Up

Once Extracted the sample needs to be cleaned of the impurties that can hinder downstream sample analysis. We offer the full Clean Up workflow, from Dialysis to Desalting and Concentration.

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General Dialysis Supplies

Desalting Columns

Protein Concentration

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Step 3: Purification

Purification is another necessary protein biology method and involves separation by protein binding, washing, and elution. The protocol used will depend on the protein you’re purifying. To get started, shop prepacked columns, products for protein purification, dialysis and desalting, and Endotoxin removal. For further information, download our resources.

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Protein Purification Reagents and Kits

Ion Exchange Columns

Magnetic Affinity Separation Particles

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Pierce protein and peptide assay selection guide (5Mb)

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Step 4: Purity Analysis

Post purification it is important to analyse the protein product. These techniques include various forms of chromatography, electrophoresis and spectrophotometry.

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Precast Electrophoresis Gels

UV-Vis Spectrophotometers

Gel Electrophoresis Reagents and Buffers

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Step 5: Protein Detection

Western blotting and ELISA techniques are frequently used for protein detection. Fluorescence imaging microscopy is also common. Browse related reagents, substrates, and imaging systems to help advance your protein detection protocol.