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Thermo Scientific™ 1-Step Human High-Yield IVT Kit
Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits.
Brand: Thermo Scientific™ 88890
This item is not returnable.
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Description
The Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits with continuous-feed device enables the expression of functional proteins with 10 to 100 times greater yield per mL than other eukaryotic IVT translation systems.
The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL. The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector.Highlights:
High expression – up to 750μg/mL of expressed protein
Reproducibility – low variability between experiments
Fast – express high levels of protein with an overnight incubation
Functional – obtain more active protein than with bacterial protein expression systems
1-Step Protein Expression:
Streamlined reaction – requires only five components
Easy-to-use – transcription and translation is performed in one reaction step
Adapt for labeling – amenable to incorporation of heavy or unnatural amino acids
Includes:
Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes
Requires:
30°C incubator or water bath
Recommended for:
Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production
Specifications
| 1-step Human High-yield Mini IVT Kit | |
| 2 x 100 μL | |
| Cell-Free |
| 2 Reactions of 100 μL Each | |
| Sufficient For: 2 reactions of 100 μL each • HeLa Lysate, 110 μL • Accessory Proteins, 25 μL • Reaction Mix, 40 μL • 5X Dialysis Buffer, 600 μL • Positive Control DNA (pCFE-GFP), 10 μg • pT7CFE1-NHis-GST-CHA, 10 μg • Microdialysis Device, 2 each • Nuclease-free Water, 5 mL Store micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C. |
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| Insoluble Protein |
For Research Use Only. Not for use in diagnostic procedures.