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Invitrogen™ Power SYBR™ Green Cells-to-C_T™ Kit

Ambion™ Power SYBR™ Green Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Brand: Invitrogen™ 4402955

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Product Code. 10259494

5550.00 EUR / Each

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Description

Ambion Power SYBRGreen Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results. A breakthrough cell lysis and RNA stabilization technology eliminates the laborious and time-consuming RNA purification process completely. Power SYBRGreen Cells-to-C_T Kit is Powered by AmpliTaq Gold™ DNA Polymerase, LD, which provides the highest levels of specificity with standard real-time PCR.

Includes optimized reagents to work efficiently, right out of the box
Simple, effective Cells-to-C_T technology enables sample preparation at room temperature in only 7 minutes, including DNase treatment
Validated accuracy, exceptional reproducibility, and maximal sensitivity from 10 to 100,000 cells per sample; results equivalent to those from purified RNA

Extraordinary Value
The kit includes reagents for sample preparation integrated into a complete, optimized gene expression workflow including reverse transcription reagents and a high-performance PCR master mix with SYBRGreen.

Extraordinary Ease
The protocol begins with a simple 7-minute sample preparation procedure. First, cells are washed in PBS, and then lysed for 5 minutes at room temperature; DNase treatment can be performed concurrently. The lysis reaction is stopped, and the sample is then ready for reverse transcription or storage at -20°C for up to 5 months. Because samples are processed directly in culture plates (96- or 384-well), sample handling and the potential for sample loss or transfer error are minimized, resulting in higher reproducibility. Following sample preparation, a portion of the cell lysate is added to an RT reaction followed by real-time PCR using the included master mix.

Extraordinary Performance
To demonstrate the performance of the kit, replicate experiments were run using either traditional RNA purification and real-time PCR analysis, or the Power SYBRGreen Cells-to-C_T Kit. The kit shows equivalent performance to results obtained using purified RNA for analysis of 10 to 100,000 cells. In addition, the sensitivity, efficiency, and dynamic range were shown to be superior to competitor lysate kits. Samples prepared with the kit have been tested for compatibility on various real-time PCR platforms including those from Applied Biosystems, Roche, and Bio-Rad.

PCR Method: 2-Step qRT-PCR
Purity/Quality Grade: LD (Low DAN)
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold™ DNA Polymerase

Accessory Product:
The SYBRGreen Cells-to-C_T Control Kit (Cat. No. 4402959) contains a Xeno™ RNA control and primer set and is recommended to monitor the efficiency of cell lysis and amplification inhibition in Cells-to-C_T reactions.

Order Info

Shipping Condition: Dry ice

Specifications

Content And Storage	Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™ RT Buffer, PowerSYBR™ Green PCR Master Mix at 4°C. Store Nuclease-free Water at room temperature
Polymerase	AmpliTaq Gold DNA Polymerase
For Use With (Equipment)	7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Label or Dye	SYBR Green
Product Line	Cells-to-CT™, SYBR™
Purity or Quality Grade	LD (Low DNA)
Reverse Transcriptase	M-MLV
For Use With (Application)	RT-PCR
Hot Start	Built-In Hot Start
Sample Type	Cells

Detection Method	SYBR
Reaction Speed	Standard
Green Features	Fewer resources used and less waste, Less hazardous
Passive Reference Dye	ROX (Pre-mixed)
Purification Time	5 min.
Quantity	400 reactions
Shipping Condition	Dry Ice
Concentration	20X
No. of Reactions	400 reactions

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Documents

Product Information

Manuals

Quick Reference Card: Fast SYBR® Green Cells-to-CT™ Kit (English ) Version: 10 Mar 2011 File size: 219.2 kB

Protocol: Power SYBR® Green Cells-to-CT™ Kit (English ) Version: 10 Mar 2011 File size: 1.2 MB

Protocol: Fast SYBR® Green Cells-to-CT™ Kit (English ) Version: 10 Mar 2011 File size: 1.2 MB

Product Information Sheet: SYBR Green Cells-to-CT™ Control Kit (English ) Version: 01 Mar 2011 File size: 117.6 kB

Instruction Manual: Power SYBR® Green Cells-to-CT™ Kit Quick Reference Card Version: 4405658 Rev B File size: 226.8 kB

Specification Sheets

Brochures & Specifications: SYBR® Green Cells-to-CT™ Kits File size: 1.1 MB

Scientific Resources

Brochures

Green Fact Sheet: Ambion Cells-to-CT Kits - Green Fact Sheet File size: 431.6 kB

Certificates

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Lot Number

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Lot Number	Certificate Type	Date	Catalog Number
Lot Number2807627	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4402955
Lot Number2800824	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4402955
Lot Number2827930	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4402955
Lot Number2821632	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4402955
Lot Number2814457	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4402955

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