missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Modifying Enzymes
  5. 10301375

Invitrogen™ Ambion™ RNase III

Double-stranded RNA (dsRNA) specific endoribonuclease

Brand:  Invitrogen™ AM2290

Product Code. 10301375

  • 496.00 EUR / Each

Please to purchase this item. Need a web account? Register with us today!

Edge
Explore more special offers
This item is not returnable. View return policy

Description

Description

Escherichia coli Ribonuclease III (RNase III; EC 3.1.24) is specific endoribonuclease. In E. coli, RNase III cleaves ribosomal RNA (rRNA) precursors during maturation of rRNA. Enzyme cleaves dsRNA into 12–15 bp dsRNA fragments with 2 to 3 nucleotide 3' overhangs, and 5' phosphate and 3' hydroxyl termini. Termini and overhangs of RNase III cleavage products are thus same as those produced by Dicer in eukaryotic RNAi pathway. Transfection of RNase III cleavage products can be used to induce RNAi in mammalian cells (patent pending).

  • Unit Definition: One unit is defined as amount of enzyme catalyzing the cleavage of 1μg of 500 bp dsRNA substrate to approximately 12–30 bp fragments in 60 min. at 37°C

DNA & RNA Purification & Analysis, In Vitro Transcribed siRNA & Dicer siRNA, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis & Blotting, RNAi, RNAi, Epigenetics & Noncoding RNA Research

Order Info

Shipping Conditions: Dry ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

RNase III and 10X Reaction Buffer should be stored at –20°C.
RNase
250 units
Ambion™
Dry Ice
10X Reaction Buffer
RNase III
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2792401 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberAM2290
Lot Number2828984 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberAM2290
Lot Number2828966 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberAM2290
Lot Number2806073 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberAM2290
Lot Number2846930 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberAM2290
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Invitrogen™ Ambion™ RNase III > 250 units

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.