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Thermo Scientific™ B-PER™ with Enzymes Bacterial Protein Extraction Kit
Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.
Specifications
| Product Line | B-PER™ |
|---|---|
| Description | B-PER Bacterial Protein Extraction Kit |
| Format | Liquid |
| Product Type | Bacterial Protein Extraction Kit |
| Product Code | Brand | Description | Quantity | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Description | Quantity | Price | Quantity & Availability | ||||
|
10405105
|
Thermo Scientific™
90078 |
B-PER Bacterial Protein Extraction Kit | 250 mL |
N/A
|
N/A | ||||
|
11746456
|
Thermo Scientific™
90079 |
B-PER Bacterial Protein Extraction Kit | 500 mL |
N/A
|
N/A | ||||
Description
Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific™ B-PER Bacterial Protein Extraction Reagents.
These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation. Certain formulations include DNase I and lysozyme enzyme supplements to improve yield of large molecular weight proteins that are difficult to purify. B-PER Reagents are compatible with the addition of standard protease inhibitors and with downstream protein purification methods, such as GST purification and His-tagged immobilized metal affinity chromatography (IMAC).Highlights:
Ready to use – B-PER Reagents provide one-step E.coli cell lysis by a mild, nonionic detergent (proprietary) in your choice of Tris or phosphate buffer formulations
Fast and simple – just add B-PER Reagent to a bacterial pellet, shake for 10 minutes and recover soluble proteins after pelleting the cell debris
Excellent yields – recover both soluble and insoluble recombinant protein from bacterial lysates and purify inclusion bodies to near-homogeneous levels
Flexible – B-PER Reagents are suitable for any scale of protein extraction and are available in phosphate and 1X and 2X Tris formulations, with and without enzymes
Compatible – completely compatible with addition of protease inhibitor cocktails, and resulting protein extract can be used in protein assays, typical affinity purification methods (e.g., GST, 6xHis) and other applications
Convenient, ready-to-use formats:
- B-PER and B-PER II (2X) Reagents are free of enzymatic components
- B-PER Reagent (in phosphate buffer) is an amine-free formulation for direct compatibility with labeling and crosslinking
- B-PER with Enzymes and B-PER Direct with Enzymes include lysozyme and DNase I for improved lysis and recovery
Includes:
Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
Specifications
| B-PER™ | |
| B-PER Bacterial Protein Extraction Kit | |
| Liquid | |
| Bacterial Protein Extraction Kit |
For Research Use Only. Not for use in diagnostic procedures.