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BD Biosciences™ DAPI Solution (RUO)

Nucleic acid stain that binds to A-T rich regions of DNA along the minor groove.

Brand:  BD Biosciences™ 564907

Product Code. 15823959

  • 94.40 EUR / Each
Estimated Shipment: 29-01-2025
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Description

Description

  • Nucleic acid stain that binds to A-T rich regions of DNA along the minor groove.
  • DAPI is predominantly impermeant to live cells, allowing it to be used as a viability dye in unfixed cells to discriminate intact from membrane-compromised cells.
  • Note, however, that high concentrations of the dye may still enter intact cells.
  • Additionally, DAPI may be used to analyze DNA content in fixed cells, or as a nuclear counterstain in imaging or flow cytometry.
  • When bound to double-stranded DNA, DAPI has an excitation wavelength maximum of 358 nm and an emission maximum of 461 nm.
  • DAPI is also well excited by the violet laser line (eg, 405 nm). Note that DAPI also binds RNA.
  • Under these conditions, DAPI emits maximally at 500 nm, but with less intensity than when bound to double-stranded DNA.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

1.0 mg/mL
Flow cytometry, bioimaging, immunofluorescence
Avoid multiple freeze-thaws of product. The product should be kept undiluted at -20°C for long term storage, and it may be kept undiluted at 4°C for short term storage.
Nucleic acid stain
1 mg
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