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Thermo Scientific™ DH10B Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
Brand: Thermo Scientific™ EC0113
115.34 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
- Suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently
- These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors
- The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal
- High transformation efficiency: >1x109 cfu/μg pUC19 DNA
- Suitable for routine and high-throughput cloning applications
- Allows for blue/white colony screening
- Convenient two reactions per vial packaging
- S.O.C. Outgrowth medium included
- Suitable for variety of applications requiring high transformation efficiency:
- Efficient DNA cloning both of prokaryotic and eukaryotic origin
- Cloning of large plasmids and BACs
- Ideal for generation of cDNA libraries using plasmid-derived vectors
- Site directed mutagenesis
- Genotype: F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(StrR) nupG
Note: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542)
Specifications
| Chemically Competent | |
| Dry Ice (-80°C) | |
| E. coli | |
| Cloning |
| Lacks F' Episome | |
| DH10B | |
| Tube | |
| 20 Reactions |