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Thermo Scientific™ DyLight™ 650 Maleimide

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of maleimide-activated DyLight Dyes.

Brand:  Thermo Scientific™ 62295

301.72 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.


Product Code. 10621601

  • 408.00 EUR / 1mg

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Description

Description

Thermo Scientific™ Sulfhydryl-Reactive DyLight Dyes are maleimide-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as probes for cellular imaging and other fluorescence detection methods.

The Thermo Scientific™ DyLight™ Dyes have absorption spectra ranging from 350 to 770nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor™ or Cy™ Dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight Reagents allows a high fluor-to-protein ratio without precipitation during conjugation.
The sulfhydryl-reactive dyes contain maleimide groups that react predominantly with free –SH groups at pH 6.5-7.5, forming a stable thioether bond. At pH 7, the maleimide group is ∼1000 times more reactive toward a free sulfhydryl than to an amine.1At pH values > 7.5, reactivity toward primary amines increases and hydrolysis of the maleimide group can occur.

Highlights:
  • Specific — maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
  • Efficient labeling methods — well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
  • Optimized antibody labeling procedure —complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:
  • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
  • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies
TRUSTED_SUSTAINABILITY
Specifications

Specifications

DyLight 650 Maleimide
Dye
Store desiccated at -20°C. Shipped at ambient temperature.
Maleimide
DyLight Fluors
672
1 mg
DMF (Dimethylformamide)
Thiol
DyLight™ 650
DyLight™
652
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot NumberZJ4483653 Certificate TypeCertificate of Analysis Date18/10/2024 Catalog Number62295
Lot NumberZA41668516 Certificate TypeCertificate of Analysis Date27/01/2024 Catalog Number62295
Lot NumberZA41706911 Certificate TypeCertificate of Analysis Date25/01/2024 Catalog Number62295
Lot Number41522216 Certificate TypeCertificate of Analysis Date25/01/2024 Catalog Number62295
Lot NumberYL41435519 Certificate TypeCertificate of Analysis Date25/01/2024 Catalog Number62295
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