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IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion
Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA Lifesciences GmbH E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli.
Brand: IBA Lifesciences 5-4143-001
This item is not returnable.
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Description
- The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
- The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
- The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
- Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.
Specifications
Inducible with anhydrotetracycline | |
Tet | |
Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage | |
His Tag (6x), Twin-Strep-tag | |
250 ng/μL | |
5 μg | |
Vector for recombinant expression in E. coli |
Ampicillin | |
Vector | |
E.coli | |
Direct Cloning | |
Forward | |
pASG-IBA143 |
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