missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Custom Products and Services
  4. Custom Gene Expression Products
  5. 15313836

Invitrogen™ Epi5™ Episomal iPSC Reprogramming Kit

Epi5™ Episomal iPSC Reprogramming Kit

Brand:  Invitrogen™ A15960

Product Code. 15313836

  • 1302.00 EUR / 1 set

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Description

Description

The Epi5™ Episomal iPSC Reprogramming Kit provides an easy-to-use, highly efficient set of 5 episomal vectors designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University. This system produces transgene-free, virus-free human induced pluripotent stem cells (iPSCs) with efficiencies in the range of 0.04% to 0.3%, depending upon the cell type being reprogrammed.

Features of the Epi5™ Episomal iPSC Reprogramming Kit include:

  • Easy to use and highly efficient—no need for small molecules during reprogramming and the added p53 suppression provides enhanced iPSC generation (1)
  • Usable for all stages of your iPSC research—transgene-free and viral-free reprogramming allows use from basic to pre-clinical research
  • Flexibility in media systems—can be used in either feeder-free or feeder-based media systems

    Create Transgene and Virus-free iPSCs
    Episomal vectors are a well-described system for producing transgene-free, virus-free iPSCs, providing a source of iPSCs for all stages of your pluripotent stem cell research. Other reprogramming methods, such as lentivirus, contain transgenes that can integrate into the host genome, potentially disrupting the genome or causing unpredictable results.

    As oriP/EBNA1 vectors, these episomal vectors contain 5 reprogramming factors (Oct4, Sox2, Lin28, Klf4, and L-Myc) and replicate extra-chromosomally only once per cell cycle. At this replication rate, the episomes are lost at a rate of approximately 5% per cell generation. This system shows enhanced iPSC generation through p53 suppression, and the inclusion of L-Myc has been shown to be more potent and specific then c-Myc during human iPSC generation (1).

    Easy-to-Use System
    This 2-vial kit provides the optimal system for episomal vector reprogramming and eliminates the need for use of small molecules in the reprogramming medium. The episomal vectors are introduced into the cell by electroporation. We recommend use of the Neon™Transfection System for electroporation, allowing flexibility in the volume and thus number of cells needed for reprogramming.

    Flexibility in Selection of Media Systems
    The Epi5™ Episomal iPSC Reprogramming Kit can be used with multiple cell types and media systems. Protocols for fibroblasts and CD34+ blood cells are available in either feeder or feeder-free based reprogramming.

    1. Okita K, Matsumara Y, Sato Y, et al. A more efficient method to generate integration free human iPS cells. Nature Methods 8, 409-412, 2011.
    TRUSTED_SUSTAINABILITY
    • Specifications

    Specifications

    None
    Transfection
    Electroporator, Neon Transfection System
    Epi5™
    CAG
    1 kit
    None
    EBNA OriP
    2 tubes. Store at -5 to -20°C.
    Stem Cell Engineering & Reprogramming
    el
    Episomal iPSC Reprogramming Kit
    None
    pUC Origin
    None
    Product Suggestions

    Product Suggestions

    Videos
    SDS
    Documents

    Documents

    Certificates
    Special Offers

    Special Offers

    Product Content Correction

    Your input is important to us. Please complete this form to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

    For Research Use Only. Not for use in diagnostic procedures.