missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ Exonuclease I (20 U/μL)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
Brand: Thermo Scientific™ EN0581
74.67 EUR valid until 2025-03-29
Use promo code "24111" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price
Description
Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.
Highlights
- Active in PCR buffers
Applications
- Primer removal from PCR mixtures:
- prior to PCR product sequencing li>for one-tube "megaprimer" PCR mutagenesis
- Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
- Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus
Note
The enzyme is not suitable for removing 3'-overhangs of dsDNA.
Specifications
| 20 U/μL | |
| Exonuclease | |
| 4,000 units | |
| Exonuclease I |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Exonuclease I
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
Supplemental information
- MIXTURE LIST-Contains : Potassium hydroxide
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Thermo Scientific™ Exonuclease I (20 U/μL) > 20μ/μL, 4000U
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction