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Thermo Scientific™ GeneBLAzer™ GPR54-NFAT-bla CHO-K1 Cells

GeneBLAzer™ GPR54-NFAT-bla CHO-K1 cells contain the human KiSS1 receptor (GPR54) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.

Brand: Thermo Scientific™ K1720

Product Code. 10686414

16470.00 EUR / Each

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Estimated Shipment: 01-04-2025
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Description

GeneBLAzer™ GPR54-NFAT-bla CHO-K1 cells contain the human KiSS1 receptor (GPR54) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1534) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

The GeneBLAzer™ GPR54-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC₅₀ concentrations of Metastin. In addition, GeneBLAzer™ GPR54-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The GPR54 receptor has been shown to be involved in tumor progression and metastasis, and in the development and functional regulation of the gonads.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Specifications

Accession Number	NP_115940.1
Cell State	Dividing Cells
Description	GeneBLAzer™ GPR54-NFAT-bla CHO-K1 Cells, 2 x 106 Cells, ∽2,600,000 cells/vial at 2,600,000 cells/mL Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
For Use With (Application)	FRET
Includes	GeneBLAzer™ GPR54-NFAT-bla CHO-K1 cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ GPR54-NFAT-bla CHO-K1 cells, GeneBLAzer™ GPR54-NFAT-bla CHO-K1 Assay Protocol, Certificate of Analysis
Product Line	GeneBLAzer™
Reporter Gene	BLA (Beta-Lactamase)
Target Entry	GPR54
Cell Line	CHO-K1
Druggable Target	GPCR

Assay Entry	Cell-based beta lactamase reporter gene
Content And Storage	LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • GeneBLAzer™ GPR54-NFAT-bla CHO-K1 cells • GeneBLAzer™ GPR54-NFAT-bla CHO-K1 Assay Protocol • Certificate of Analysis The GeneBLAzer™ GPR54-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method	Fluorescence
Gene Symbol	GPR54
Population Status	Single Cell Clone Origin
Quantity	2 x 10⁶ Cells
Sample Type	CHO-K1
Type	CHO-K1 Cells
System Type	GeneBLAzer™

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Documents

Product Information

Manuals

User Guide: GeneBLAzer GPR54‑NFAT-bla CHO‑K1 Cell–Based Assay Version: 2.0 File size: 390.5 kB

GeneBLAzer® GPR54 NFAT-bla CHO-K1 Cell Line Validation Packet Version: version 1.1 File size: 90.4 kB

Certificates

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Lot Number

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Lot Number	Certificate Type	Date	Catalog Number
Lot Number990138	Certificate TypeCertificate of Analysis	Date15/04/2017	Catalog NumberK1720

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