missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Culture Media, Supplements, and Reagents
  4. Cell Culture Media
  5. Classical and Basal Cell Culture Media
  6. Ham's F-12 Cell Culture Media
  7. Gibco™ Ham's F-12 Nutrient Mix

Gibco™ Ham's F-12 Nutrient Mix
GREENER_CHOICE

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.

26.80 EUR - 47.23 EUR

See promotional offers below

Specifications

Cell Line CHO, COS-7, and rat prostate epithelial cells
Cell Type Primary Rat Astrocytes
Classification Animal Origin-free
Concentration 1 X
Form Liquid
View More Specs

Requires: Supplementation


Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15172529
GREENER_CHOICE
View Documents Promotion Details
missing translation for 'viewProductCertificates'
Gibco™
11765054 		500 mL
47.23 EUR
500mL
Please to purchase this item. Need a web account? Register with us today!
15440564
GREENER_CHOICE
View Documents Promotion Details
missing translation for 'viewProductCertificates'
Gibco™
11765047 		1000 mL
26.80 EUR
1000mL
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:

With: L-glutamine, Phenol Red

The complete formulation is available.

Using F-12

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

CHO, COS-7, and rat prostate epithelial cells
Animal Origin-free
Liquid
Sterile-filtered
No HEPES
Gibco™
Primary Rat Astrocytes
1 X
Ham's F-12 Nutrient Mixture
Glutamine, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
Videos
Safety and Handling

Safety and Handling

missing translation for 'shelfLife' : 12 Months From Date of Manufacture

SDS
Documents

Documents

Product Certifications
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.