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Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Brand: Invitrogen™ 10342020
Includes: Taq DNA Polymerase (5U/μL); 10X PCR buffer (200mM Tris-HCl pH 8.4, 500mM KCl); Magnesium Chloride (50mM)
Description
Taq DNA Polymerase, recombinant, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.
Features
- A choice of recombinant or native enzyme
- Amplification of PCR products up to 5 kb in size
- Enzyme is licensed and qualified for PCR
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- Sequencing ssDNA
- Cycle sequencing
Notes
- One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
- For superior PCR performance, DreamTaq DNA Polymerase is recommended.
Order Info
Shipping Condition: Approved for shipment on wet or dry ice
Specifications
| • Taq DNA Polymerase (5 U/μL), 100 μL • 10X PCR buffer, 2.5 mL • 50 mM MgCl2, 1 mL Store at -10°C to -30°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. |
|
| Low | |
| Taq DNA Polymerase | |
| 5' - 3' | |
| 500 Units | |
| Standard PCR | |
| 1X | |
| 500 Reactions | |
| Separate Components | |
| DNA |
| Stand-alone enzyme | |
| qPCR, Standard PCR | |
| Standard | |
| DNA Polymerase | |
| Dry Ice | |
| 5 U/μL | |
| No | |
| 3'-A | |
| 5 kb or less |
For Research Use Only. Not for use in diagnostic procedures.