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Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit

The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli.

218.00 EUR - 510.00 EUR

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Specifications

Format	Column
Isolation Technology	Anion Exchange Resin
Scale	Maxi
Sample Type	Bacterial Culture
Elution Volume	15 mL

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Description

The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 μg plasmid DNA from 100–200 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
High-purity, low-endotoxin product- pure enough for mammalian cell transfection
Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification

PureLink HiPure Plasmid DNA Purification Kits use patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
Resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal
Plasmid preparations can typically be completed in less than 2 hours

Contaminant-free DNA

Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A₂₆₀/A₂₈₀ ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
Purification time is two hours

Order Info

Shipping Condition: Room Temperature

Specifications

Format	Column
Scale	Maxi
Elution Volume	15 mL
For Use With (Application)	Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Plasmid	<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Product Line	PureLink™
Purity	Transfection Grade
Target	BAC DNA, Plasmid DNA
Test Time	2 hr.

Isolation Technology	Anion Exchange Resin
Sample Type	Bacterial Culture
Final Product Type	BAC DNA, Plasmid DNA
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Prep Scale	> 200 μg (Large-Scale) Plasmid DNA
Product Type	Plasmid MaxiPrep Kit
Shipping Condition	Room Temperature
System Type	PureLink™
Yield	1000 μg

Safety and Handling

Product Identifier

PureLink HiPure Plasmid Maxiprep Kit

Signal Word

Warning

Hazard Category

Serious eye damage/eye irritation Category 2
Skin corrosion/irritation Category 2

Hazard Statement

H315-Causes skin irritation.
H319-Causes serious eye irritation.

Precautionary Statement

P264-Wash hands thoroughly after handling.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P332+P313-If skin irritation occurs: Get medical advice/attention.
P337+P313-If eye irritation persists: Get medical advice/attention.
P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information

MIXTURE LIST-Contains : acetic acid

For Research Use Only. Not for use in diagnostic procedures.

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Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit

Specifications

Description

Order Info

Specifications

Safety and Handling

Documents