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Thermo Scientific™ Kinase Tracer 236, 50 μM

In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 236 to a kinase is detected by addition of a Eu-labeled anti-tag antibody.

Brand: Thermo Scientific™ PV5592

Product Code. 10529763

460.00 EUR / Each

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Description

In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 236 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.

Specifications

Assay	Kinase Binding Assay
Content And Storage	Store at -20°C.
For Use With (Equipment)	Microplate Reader
Label or Dye	Alexa Fluor™ 647
Readout	Kinetic
Target Entry	CSF1R, CSK, EPHA5, PDGFRA T674I, PDGFRA V561D, RET Y791F, SYK, TYRO3, ZAP70, MAP4K5, PRKCQ, PRKX, ROCK2, IKBKE, PAK4, MAP3K2, LRRK2 D1994A, GRK7, GSK3A, GSK3B, SNF1LK2, MAP2K1 S218D S222D, FGFR1, FGFR2, MAP3K10, MAP3K9, JAK2 JH1 JH2, AMPK (A2/B1/G1), CAMK2B, CHEK1, PIM2, PRKCN, PRKD2, PRKG2, RPS6KA5, MELK, BLK, EPHB2, IGF1R, LYNA, MERTK, RET V804L, ROS1, TEK, TTK, SGK, CHUK, IKBKB, TAOK3, TYK2, IRAK1, MYLK2, SRPK2, STK4, FLT1, MUSK, CAMK2D, LRRK2 R1441C, DYRK1A, DYRK3, MAP2K1, MAP2K6, RPS6KA6, BMX, EPHA4, FYN, PTK2, PTK2B, RET, MAP4K2, MINK1, GRK6, JAK3, LRRK2, AMPK (A1/B1/G1), AURKA, CAMK1D, CAMK4, PIM1, PKN1, PRKD1, PRKG1, RPS6KA4, STK22D, NUAK1, EPHA3, FLT3, FLT4, INSR, LCK, YES1, KDR, MET, TNK2, PRKCI, CAMK2A, RPS6KA3, MAP3K3, ABL2, AXL, FGFR3, FGFR3 K650E, FGR, HCK, AURKB, TAOK2, MAP3K11, CAMKK1, CDC42BPA, CDK1, CDK5, CDK8, CDK9/Cyclin K, MAP2K3, CSNK1G3, DMPK, DYRK1B, ACVR1, EPHA1, EPHA8, LYNB, MST1R, PDGFRA D842V, MAP4K4, LRRK2 G2019S, CAMKK2, CDC42BPB, CDK2, CDK7, CDK9/Cyclin T1, EPHA2, FER, LTK, PDGFRA, PDGFRB, MARK4, RPS6KB1, EGFR T790M, EPHA7, EPHB1, PHKG1, ROCK1, RPS6KA1, TBK1, JAK2, MARK1, PHKG2, MLCK, PAK6, SLK, LIMK1, MKNK2, MYLK, FRK, ITK, LRRK2 I2020T, BRSK1, CLK2, PDK1, MAP2K2, MAP3K14, MAP2K6 S207E T211E, FLT3 D835Y, SRC, GRK5, GRK4, STK3, BTK, FES, KIT T670I, RPS6KA2, SGKL, STK25, PAK7, PRKACA, CLK1, CLK4, MARK2, STK17A, PRKCH, EIF2AK2, MAP3K5, LIMK2, STK16

Assay Entry	Biochemical binding/kinetic
For Use With (Application)	TR-FRET
Format	384-well Plate
Quantity	25 μL
Shipping Condition	Dry Ice
Type	Kinase Tracer

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Documents

Scientific Resources

Posters

Determination of drug-kinase residence time in a homogeneous, low-volume format File size: 665.5 kB

Competitive TR-FRET binding assays for PI3K family inhibitors File size: 791.1 kB

Characterization of Kinase inhibitors with versatile, homogenous, competitive TR-FRET binding assays File size: 746.5 kB

Characterization of both allosteric and ATP-competitive kinase inhibitors with TR-FRET binding assay File size: 238.4 kB

A flexible kinase inhibitor assay for active, nonactivated, and impure kinase preparations File size: 632.8 kB

Brochures

Use of LanthaScreen® Eu Kinase Binding Assay for Type III Kinase Inhibitors File size: 445.2 kB

Binding Assay Kinase Selectivity Data File size: 33.6 kB

CO21197 Assay Dev Ap Note LRF.pdf File size: 711.3 kB

Simple Off-rate Measurements Application Note File size: 469.3 kB

Product Information

Manuals

Instrument setup guide- Paradigm- LanthaScreen Eu Version: 10.3.2012 File size: 769.2 kB

Eu Instrument Control Application Note Version: N/A File size: 216.3 kB

LanthaScreen Kinase Binding Assay User Guide_man Version: 16 April 2010 File size: 229.2 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number3019843A	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberPV5592
Lot Number2796262E	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberPV5592
Lot Number3019843D	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberPV5592
Lot Number2796262C	Certificate TypeCertificate of Analysis	Date28/12/2023	Catalog NumberPV5592
Lot Number2623465H	Certificate TypeCertificate of Analysis	Date30/09/2023	Catalog NumberPV5592

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