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Alexa Fluor™ Kinase Tracer 314, 25 μM
Alexa Fluor™ Kinase Tracer 315 is detected by addition of a Eu-labeled anti-tag antibody.
Brand: Alexa Fluor™ PV6087
Description
- Excitation/Emission (nm): 340/615, 665
- Label or Dye: Alexa Fluor™ 647
- Target Enzyme: Kinases (Multiple)
- Validated Kinases: PIK3CD, PIK3CG, FRAP1 (mTOR)
- In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 314 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.

Specifications
Kinase Binding Assay | |
Store at -20°C. | |
TR-FRET | |
384-well Plate | |
25 μL | |
Dry Ice | |
Kinase Tracer |
Biochemical binding/kinetic | |
340/615, 665 | |
Microplate Reader | |
Alexa Fluor™ 647 | |
Kinetic | |
PIK3CG, PIK3CD/PIK3R1, mTOR, FRAP1 | |
PIK3CD, PIK3CG, FRAP1 (mTOR) |
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Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Catalog Number |
---|---|---|---|
2834671E | Certificate of Analysis | 25/03/2025 | PV6087 |
2834671D | Certificate of Analysis | 21/02/2025 | PV6087 |
2834671C | Certificate of Analysis | 21/02/2025 | PV6087 |
2834671B | Certificate of Analysis | 21/02/2025 | PV6087 |
2834671A | Certificate of Analysis | 04/04/2024 | PV6087 |
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