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MP Biomedicals™ Lysozyme, MP Biomedicals™
Used for lysing E. coli and Streptomycetes for extraction purposes. Lysozyme, MP Biomedicals preferentially hydrolyzes the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. Enzyme activity: ≥20,000u/mg solid.
Brand: MP Biomedicals™ 02100831.5
This item is not returnable.
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Description
An enzyme used in a variety of biochemical applications.
- For lysing E. coli and Streptomycetes for extraction purposes, such as extracting group specific antigen
- May act as a germinative agent of bacterial spores
- In humans, may be the mediator in the anti-tumor function of macrophages which secrete the enzyme
- There is evidence that cartilage lysozyme has a role in cartilage calcification
- The enzyme breaks down the cell walls of bacteria
- Used to prepare spheroplasts
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.
- These links occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus.
- Gram-positive cells are quite susceptible to this hydrolysis, as their cell walls have a high proportion of peptidoglycan.
- Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
- The enzyme is active over a broad pH range (6.0–9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02–0.100M) than at pH 9.2 (0.01–0.06M).
Specifications
| Greater than or equal to 20,000 U/mg | |
| Lysozyme | |
| 2235.597 g/mol | |
| Store at -20°C | |
| Powder | |
| 500 mg |
| White | |
| 9001-63-2 | |
| Muramidase; Mucopeptide N-acetylmuramoylhydrolase | |
| Chicken Egg White | |
| Cell Lysis |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Lysozyme
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- Skin sensitiser Category 1
Hazard Statement
- H302-Harmful if swallowed.
- H317-May cause an allergic skin reaction.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P321-Specific treatment (see on this label).
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : lysozyme