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Invitrogen™ Rhod-5N, Tripotassium Salt, cell impermeant

Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipet, microinjection or pinocytotic cell-loading reagent

Brand:  Invitrogen™ R14207

Product Code. 11563677

  • 467.00 EUR / 500µg

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Description

Description

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+ release generated by photoreceptors and photoactivatable chelators.

  • Fluorescence signal from these cells is generally measured using fluorescence microscopy
  • Fluorescence intensity increase upon binding Ca2+: >100 fold
  • Kd for Ca2+ in absence of Mg2+, in buffer: approximately 320μM
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
  • In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
  • Perturbations to calcium measurements caused by presence of these ions can be controlled using heavy metal-selective chelator TPEN

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Store in freezer -5°C to -30°C and protect from light.
Fluorescence Microscope
Fluorescent Dye-Based
Room Temperature
Fluorescence
Calcium Indicator
500 μg
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2961123 Certificate TypeCertificate of Analysis Date04/06/2024 Catalog NumberR14207
Lot Number2747488 Certificate TypeCertificate of Analysis Date02/12/2023 Catalog NumberR14207
Lot Number2299800 Certificate TypeCertificate of Analysis Date02/03/2021 Catalog NumberR14207
Lot Number2286837 Certificate TypeCertificate of Analysis Date23/12/2020 Catalog NumberR14207
Lot Number2251158 Certificate TypeCertificate of Analysis Date19/08/2020 Catalog NumberR14207
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Invitrogen™ Rhod-5N, Tripotassium Salt, cell impermeant > 500ug

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For Research Use Only. Not for use in diagnostic procedures.