Thermo Scientific™ Bacillus Cereus Enterotoxin Reversed Passive Latex Agglutination Kit (BCET – RPLA)

Description

Rapidly detect Bacillus cereus enterotoxin (diarrhoeal type) in foods and culture filtrates with Thermo Scientific™ Oxoid™ Bacillus Cereus Enterotoxin Reversed Passive Latex Agglutination Kit. The technique of reversed passive latex agglutination (RPLA) enables soluble antigens such as bacterial toxins to be detected in an agglutination assay.

The BCET-RPLA test may be used to detect B. cereus enterotoxin in a variety of foods and to give a semi-quantitative result. The test may also be used to demonstrate enterotoxin production by isolates of B. cereus grown in culture.

Bacillus cereus is a common inhabitant of many different environments and can easily contaminate food. If contaminated foods are not cooled sufficiently after cooking and there is an extended time between preparation and consumption of foods, then surviving heat-resistant spores can germinate, enabling the organism to multiply and produce toxins. Under these circumstances, B cereus can cause food poisoning.

Use Bacillus cereus Enterotoxin RPLA test for simple and semi-quantitative detection of staphylococcal enterotoxins in food samples.

• Simple – easy to implement
• Visual – easy to read results

In a standard agglutination assay, the soluble antibody reacts with a particulate antigen such as bacterial cells. However, in a reversed agglutination assay the antibody, which is attached to particles, reacts with the soluble antigen. The particles (in this case, latex) do not themselves play a part in the reaction and they are therefore passive. The cross-linking of the latex particles by the specific antigen/antibody reaction results in the visible latex agglutination reaction.

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