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Invitrogen™ PARP (Cleaved) Multispecies In-Cell ELISA Kit, Colorimetric

In-cell ELISA Kit

Brand:  Invitrogen™ 62219

Product Code. 11558100

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Description

Description

The Cleaved PARP Multispecies In-Cell ELISA Colorimetric Detection Kit is a simple method for quantifying intracellular proteins in whole cells. Principle of the method To perform the assay, cells are first plated, treated and fixed. Expression of the protein(s) of interest is monitored in wells of a microplate using target-specific primary antibodies (see the Important Product Information section for antibodies included in each kit) and a horseradish peroxidase (HRP)-conjugated detection reagent. The kit is supplied with a whole-cell stain to control for differences in cell plating, which is important when measuring relative levels of a protein with different treatments or assessing its post-translational modification (PTM) form. After staining, the results are analyzed by normalizing the absorbance (HRP activity) values to cell number, which adjusts for the cell plating differences among the wells. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

P09874
HRP
ELISA Kit
96 microplate wells of overnight-cultured cells
Colorimetric Microplate Reader
ADPRT,ADPRT 1,ADPRT1,ARTD1,PARP,PARP-1,PPOL,pADPRT-1
HRP
RUO
2°C to 8°C
1 hr. 20 min.
Pierce Cleaved PARP Colorimetric In-Cell ELISA Kit
Multi-component kit with HRP-conjugate
Supernatant
Human
142
Blocking Buffer, 20X Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, HRP Conjugate, TMB Substrate, TMB Stop Solution, Janus Green Whole-Cell Stain, Elution Buffer, Thin Plate Seal Assembly, Components included only in the target-specific kits:Antibody #1 and Antibody #2, Anti-Cleaved PARP Antibody
96 Tests
Supernatant, 10,000 cells
Human
24 hr.
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Invitrogen™ PARP (Cleaved) Multispecies In-Cell ELISA Kit, Colorimetric > 96 Tests

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