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Thermo Scientific™ Pierce™ Glutathione Magnetic Beads
Affinity purify GST-tagged fusion proteins from cell lysates using glutathione (GSH) magnetic particles for manual or robotic magnetic separation devices.
Brand: Thermo Scientific™ 88822
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Description
Thermo Scientific™ Pierce Glutathione Magnetic Beads are superior GST-binding particles for automated magnetic separations.
Additional Information and Online Ordering
Glutathione magnetic beads provide a simple, rapid and reliable means of purifying glutathione S-transferase (GST) fusion proteins from crude cell lysates prepared from bacteria, yeast or mammalian cells. The protocol for GST-tagged protein purification is simple: the magnetic particles are added to a lysate containing the GST-fusion protein, and after incubation, non-bound proteins are removed by washing and the GST-fusion proteins are eluted using reduced glutathione. The high-performance iron oxide superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher™ 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.Highlights:
High-performance beads – non-aggregating, silanized, iron oxide, paramagnetic microparticles provide exceptional uniformity for automated HTS applications
Stable affinity ligand – glutathione is covalently immobilized to particles, ensuring leach-resistance and clean purification products
High capacity – binding capacity is sufficient for both routine and demanding magnetic separation procedures
GST-tag affinity – standard glutathione-GST affinity purification chemistry in a magnetic format; bind in physiologic buffers and elute with 50mM reduced glutathione (prepared with Part No. 78259)
Properties of Glutathione Magnetic Beads:
- Composition: iron oxide particles with reduced glutathione (GSH) covalently attached to the surface and encapsulated by crosslinked agarose
- Magnetization: superparamagnetic (no magnetic memory)
- Bead size: 1 to 10μm (mean diameter)
- Concentration: 25% slurry (based on volume) in 20% ethanol
- Binding capacity: 5 to 10mg of GST fusion protein per milliliter of settled beads (i.e., 4mL of 25% slurry)
Requires:
Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device
Specifications
| Pierce Glutathione Magnetic Beads | |
| Magnetic Bead | |
| Binding 5 to 10mg GST per mL of beads |
| Glutathione on paramagnetic iron oxide particles (1 to 10um dia.) slurried in 20% ethyl alcohol | |
| 20 mL |
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